Mass-propagation of Virus-free Plants of Japanese Butterbur (Petasites japonicus Fr. Schmidt) by Tissue Culture.

Use of Tissue Culture for the Mass Propagation of Pathogen-free Plants

Different in vitro culture techniques have been used for rapid plant propagation. Pathogenfree plants have been produced using techniques such as seed culture, meristem culture, micropropagation using axillary or adventitious shoot buds, and somatic embryogenesis. This Bulletin discusses the importance of in vitro culture techniques for the production of pathogen-free medicinal plants such as A...

Propagation of Rabies Virus in Tissue Culture

Rabies virus has been propagated in serum-Tyrode solution containing either embryo mouse brain or embryo chick brain. The culture virus reached a titre of 3 x 10(-5) cc. after 4 days' incubation at 37 degrees C., and survived at least 2 months at 5 degrees C. in the liquid or dry state.

Adaptation of Horsesickness Virus to Tissue Culture

Petasites japonicus Stimulates the Proliferation of Mouse Spermatogonial Stem Cells

Oriental natural plants have been used as medical herbs for the treatment of various diseases for over 2,000 years. In this study, we evaluated the effect of several natural plants on the preservation of male fertility by assessing the ability of plant extracts to stimulate spermatogonial stem cell (SSC) proliferation by using a serum-free culture method. In vitro assays showed that Petasites j...

Petasites hybridus (Butterbur root) extract in the treatment of asthma--an open trial.

The efficacy and tolerability of a butterbur root extract (Petadolex) for the treatment of asthma was analyzed in a prospective, non-randomized, open trial. Subjects included 64 adults and 16 children/adolescents treated for two months with the extract, followed by two months during which the intake of the extract was optional. Concomitant asthma medication was permitted. The number, duration, ...